วิศวกรรมเอนไซม์ลูซิเฟอเรสจากแบคทีเรียเพื่อการประยุกต์ใช้เป็นตัวชี้วัดทางชีวภาพ : Engineering of bacterial luciferase enzyme for bioreporter applications

1. To construct truncated fusion Lux enzyme by engineering the ?-subunit. 2. To study and characterize truncated fusion Lux enzyme. 3. To construct secreted fusion Lux enzyme by fusing the codon-optimized lux gene with various types of signal peptides. 4. To study secretion efficiency of different types of signal peptides and stability of secreted fusion Lux in cell culture medium