การศึกษาหน้าที่ของโปรตีนที่คาดว่าจะทำหน้าที่เป็นไตรเอซิลกลีเซอรัลไลเปสในสาหร่าย Chlamydomonas reinhardtii : Characterization of a putative triacylglycerol lipase in Chlamydomonas reinhardtii

Biological significance and potential to function as a TAG lipase of CrLIP4 will be investigated through three major experiments. First, CrLIP4 gene expression pattern will be monitored along with the cellular TAG concentration under various growth conditions. Second, Growth and TAG phenotype of lip4 mutants generated through insertional mutagenesis will be analyzed. Finally, CrLIP4 genomic DNA complementation will be carried out in the lip4 mutants. The transformants will be tested their growth and lipid phenotype. If the wild type gene can rescue the mutant’s phenotypes, it is likely that the gene functions as a TAG lipase.